single cell capture  (Singleron Biotechnologies)

Journal: STAR Protocols

Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

doi: 10.1016/j.xpro.2026.104527

Figure Lengend Snippet: Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.

Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

Techniques: Plasmid Preparation, Sequencing, Virus, Single Cell

Journal: STAR Protocols

Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

doi: 10.1016/j.xpro.2026.104527

Figure Lengend Snippet: Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.

Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

Techniques: Flow Cytometry, Single Cell, RNA Sequencing, Fluorescence, Control

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: Chemokine (C-C motif) Ligand 2 Expressing Adventitial Fibroblast Expansion During Loeys-Dietz Syndrome Aortic Aneurysm Formation

doi: 10.1161/ATVBAHA.124.322069

Figure Lengend Snippet: A) Cd68 immunofluorescence staining of Tgfbr2 G357W/+ and WT aortic root. Red – Cd68, blue – Hoechst nuclear stain; green – elastin autofluorescence. Top : 10x magnification; scale bar: 100μm. Bottom : 20x magnification; scale bar: 50μm. B) UMAP projection of macrophages after reclustering into 3 clusters: MΦ1–3. C) Feature plots of cluster defining genes. MΦ1/Resident macrophages: F13a1, Lyve1, and Gas6 ; MΦ2/M1 macrophages: Cd74, H2-Aa, Cd40 ; MΦ3/M2 macrophages Arg1, Ccl24, Mrc1 . D) UMAP projection split by genotype identified similar resident clusters but substantially increased MΦ2 and MΦ3 clusters in Tgfbr2 G357W/+ . E) Bar plot by percentage and absolute cell count for each macrophage cluster by genotype. F) Feature plot of MΦ1/Resident ( F13a1 ), MΦ2/M1 ( Ccr2 ), and MΦ3/M2 ( Arg1 ) macrophage defining genes split by genotype. Very few MΦ2/M1 and MΦ3/M2 macrophages exist within the WT samples. UMAP - uniform manifold approximation and projection.

Article Snippet: Single cell suspensions were counted to ensure adequate cell density (>150 cells/μL) and immediately used for 10X Genomics single-cell capture.

Techniques: Immunofluorescence, Staining, Cell Characterization

Journal: Cell reports

Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis

doi: 10.1016/j.celrep.2025.115356

Figure Lengend Snippet:

Article Snippet: ND GBCF prepared cells for 10X Genomics Chromium single cell capture and cDNA libraries according to the standard CITE-seq ( https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf ) and 10X Genomics standard protocols.

Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence